Green fluorescent protein - Tagged HCV non-enveloped capsid like particles: Development of a new tool for tracking HCV core uptake


Katsarou K., Serti E., Tsitoura P., Lavdas A. A. , Varaklioti A., Pickl-Herk A., ...Daha Fazla

BIOCHIMIE, cilt.91, ss.903-915, 2009 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 91 Konu: 7
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.biochi.2009.04.016
  • Dergi Adı: BIOCHIMIE
  • Sayfa Sayıları: ss.903-915

Özet

Circulating 'free' non-enveloped Hepatitis C virus (HCV) core protein has been demonstrated in HCV-infected patients, and HCV subgenomes with deletions of the envelope proteins have been previously identified. Initial studies from our laboratory, previously published, indicated that expression of HCV core in insect cells can direct the formation of capsid-like particles lacking the envelope glycoproteins. These protein nanospheres, morphologically similar to natural capsids, were shown to be taken up by human hepatic cells and to produce cell-signalling events. To follow the intracellular fate of these particles we fused the core protein to eGFP. We demonstrate that the chimeric proteins core(173)-eGFP, eGFP-core(191) and eGFP-core(173) can be efficiently expressed, self-assembled, and form fluorescent non-enveloped capsid-like particles. By using confocal microscopy and FACS analysis, we provide evidence that the fluorescent nanospheres can not only enter human hepatic cells - the main target of HCV - but also human immune cells such as T and B lymphocytes, as well as human myeloid leukaemia cells differentiated along the monocyte/macrophage-like pathway The fluorescent particles might thus be used to trace the intracellular trafficking of naked HCV capsids as showed by live microscopy and to further understand their biological significance. (C) 2009 Elsevier Masson SAS. All rights reserved.