Protective effects of exercise on heart and aorta in high-fat diet-induced obese rats

AÇIKEL ELMAS M., Cakici S. E., Dur I. R., Kozluca I., Arinc M., Binbuga B., ...Daha Fazla

TISSUE & CELL, cilt.57, ss.57-65, 2019 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 57
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.tice.2019.01.005
  • Dergi Adı: TISSUE & CELL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.57-65
  • Anahtar Kelimeler: High-fat diet, Exercise, Aorta, Heart, Nitric oxide synthase, Ultrastructure, MAST-CELL ACTIVATION, OXIDATIVE STRESS, REGULAR EXERCISE, ENDOTHELIAL DYSFUNCTION, TNF-ALPHA, INJURY, TISSUE, HYPERTENSION, CHOLESTEROL, DISEASES
  • Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Evet

Özet

We investigated the protective effects of swimming exercise on high-fat diet-induced heart and aorta damage by evaluating oxidative stress and the endothelial nitric oxide (NO) system. Sprague Dawley rats were fed either standard chow (STD, 6% fat) or high-fat diet (HFD; 45% fat) for 18 weeks, with half of the animals trained by daily swimming sessions (EXC; 1 h per day for 5 days/week) for the last 6 weeks of the experimental period and half kept sedentary (SED). Heart and aorta tissues were prepared for routine light and electron microscopy evaluation. Endothelial NOS (eNOS) and inducible NOS (iNOS) distribution in the tissue samples were examined by immunohistochemistry. Biochemical examinations, including blood serum lipid profiles, malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), and tissue NO levels were measured. Deteriorated heart and aorta morphology, increased MDA levels and iNOS-immunoreactivity (iNOS-ir), as well as decreased GSH, NO, SOD, and eNOS-ir parameters were observed in the HFD+ SED group. These morphological and biochemical parameters were ameliorated in the HFD+ EXC group. Our study revealed that obesity-induced iNOS activation and increased oxidative stress in cardiac and aorta tissues. Exercise protected the obesity-induced cardiac and aortic tissue damage by modulating oxidant/antioxidant balance via involvement of the NO system.