Muscarinic agonist, antagonists and signaling pathway inhibitors change c-Fos and cyclin D-1 expression in K562 cells

CABADAK H., AYDIN OMAY B., KAN B.

MARMARA MEDICAL JOURNAL, cilt.26, sa.2, ss.72-76, 2013 (ESCI) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 2
  • Basım Tarihi: 2013
  • Doi Numarası: 10.5472/mmj.2013.02807.1
  • Dergi Adı: MARMARA MEDICAL JOURNAL
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.72-76
  • Anahtar Kelimeler: Atropine, Carbachol (CCh), c-Fos, Cyclin D-1, MESSENGER-RNA EXPRESSION, PROTEIN-KINASE-C, ACETYLCHOLINE-RECEPTORS, GENE-EXPRESSION, JUN, D1, PROTOONCOGENE, TRANSDUCTION, ACTIVATION, MECHANISMS
  • Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Evet

Özet

Objectives: Muscarinic acetylcholine receptors (mAChR) belong to a family of G protein coupled receptors (GPCRs). These mAChRs regulate several important physiological functions by activating a wide variety of cellular signaling pathways. We have previously shown that muscarinic acetylcholine (M-2, M-3 and M-4) receptors are expressed in K562 cells. In this study, we investigated the effect of muscarinic agonist, antagonists and different signaling pathway inhibitors on c-Fos and cyclin D-1 transcripts, using reverse transcriptase polymerase chain reaction (RT-PCR) that allows changes of very rare transcripts to be monitored.