Real-time, in situ DNA hybridization detection with attomolar sensitivity without amplification using (pb(Mg1/3Nb2/3)O-3)(0.65)-(PbTiO3)(0.35) piezoelectric plate sensors


Wu W., Kirimli C. E. , Shih W., Shih W. Y.

BIOSENSORS & BIOELECTRONICS, cilt.43, ss.391-399, 2013 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 43
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1016/j.bios.2012.12.044
  • Dergi Adı: BIOSENSORS & BIOELECTRONICS
  • Sayfa Sayısı: ss.391-399

Özet

In this paper we have investigated real-time, in situ DNA hybridization detection using piezoelectric plate sensors (PEPSs) consisting of a highly piezoelectric lead magnesium niobate-lead titanate (PMN-PT) layer 8 mu m in thickness thinly coated with Cr/Au electrodes and electrically insulated with 3-mercaptopropyltrimethoxysilane (MPS) encapsulation. With probe complementary DNA (cDNA) immobilized on the PEPS surface and by monitoring the first longitudinal extension mode (LEM) resonance frequency shift of the PEPS we detected hybridization of the target DNA (tDNA) to the probe cDNA on the PEPS surface in real time at concentration 1.6 x 10(-18) M with a signal to noise ratio of 8 without isolation and amplification at room temperature in 30 min in phosphate buffered saline (PBS) solution. The detection was validated in situ by two different methods: (1) the detection of fluorescently labeled microspheres coated with reporter cDNA complementary to the tDNA but different from the probe cDNA; (2) fluorescent visualization. (C) 2012 Elsevier B.V. All rights reserved.