Fiber Connections of the Supplementary Motor Area Revisited: Methodology of Fiber Dissection, DTI, and Three Dimensional Documentation


Bozkurt B., Yagmurlu K., Middlebrooks E. H., Cayci Z., Cevik O. M., Karadag A., ...Daha Fazla

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, cilt.2017, sa.123, 2017 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 2017 Sayı: 123
  • Basım Tarihi: 2017
  • Doi Numarası: 10.3791/55681
  • Dergi Adı: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Neuroscience, Issue 123, Supplementary motor area, fiber dissection, diffusion tensor tractography, three-dimensional documentation, white matter pathways, association fibers, commissural fibers, projection fibers, IN-VIVO, DISCONNECTION SYNDROMES, PRE-SMA, MOVEMENT, NETWORKS, LANGUAGE, ANIMALS, HUMANS, CORTEX
  • Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Hayır

Özet

The purpose of this study is to show the methodology for the examination of the white matter connections of the supplementary motor area (SMA) complex (pre-SMA and SMA proper) using a combination of fiber dissection techniques on cadaveric specimens and magnetic resonance (MR) tractography. The protocol will also describe the procedure for a white matter dissection of a human brain, diffusion tensor tractography imaging, and three-dimensional documentation. The fiber dissections on human brains and the 3D documentation were performed at the University of Minnesota, Microsurgery and Neuroanatomy Laboratory, Department of Neurosurgery. Five postmortem human brain specimens and two whole heads were prepared in accordance with Klingler's method. Brain hemispheres were dissected step by step from lateral to medial and medial to lateral under an operating microscope, and 3D images were captured at every stage. All dissection results were supported by diffusion tensor imaging. Investigations on the connections in line with Meynert's fiber tract classification, including association fibers (short, superior longitudinal fasciculus I and frontal aslant tracts), projection fibers (corticospinal, claustrocortical, cingulum, and frontostriatal tracts), and commissural fibers (callosal fibers) were also conducted.