30th ANNUAL BIOPHYSICS CONGRESS (INTERNATIONAL), Muğla, Türkiye, 10 - 13 October 2018, ss.1-63
Introduction: Titanium dioxide (TiO2) nanoparticles are manufactured in different size/shape and widely used in cosmetic industry (toothpaste, shampoo, sun cream etc.), biomedical implants, pharmaceutical field and as food additive (chewing gum, chocolates, white sugar etc.). Blood coagulation is an important physiological process to stop bleeding in case of tissue damage. However, if it becomes pathologic (excessive clotting) then may lead to thrombosis, stroke and myocardial infarction. It is not yet known whether TiO2 nanoparticles in the plasma system activate blood coagulation via the intrinsic pathway of blood coagulation (via factor XII). It is aimed to investigate the effects of TiO2 nanoparticles in different shape, size and concentration on blood coagulation via factor XII activation. Methods: Particles were purchased in sizes of 5, 40 and 200 nm and shape-surface analysis has been done by scanning electron microscopy (SEM). All particles were suspended in Hepes-NaCl (HN) buffer at final concentrations of 2.08, 4.2, 8.3 and 16.6 µg/ml and thrombin generation was recorded after addition into human platelet poor plasma. Results: Thrombin generation was increased dose dependently for 5nm and 200nm TiO2 nanoparticles through the intrinsic pathway. However thrombin curves for 40 nm particles at all concentrations were similar to control (buffer). Conclusion: It is concluded that TiO2 particles in certain shape activates blood coagulation with concentration and size dependent manner.
Key words: Factor XII, TiO2, Blood Coagulation, SEM, intrinsic pathway of coagulation