Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species


Akyar I. , ÇAVUŞOĞLU C., Ayas M., SÜRÜCÜOĞLU S., Ilki A., Kaya D. E. , et al.

TURKISH JOURNAL OF MEDICAL SCIENCES, cilt.48, ss.1351-1357, 2018 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 48 Konu: 6
  • Basım Tarihi: 2018
  • Doi Numarası: 10.3906/sag-1801-198
  • Dergi Adı: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Sayfa Sayısı: ss.1351-1357

Özet

Background/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis.