Phenolic compounds from the aerial parts of Clematis viticella L. and their in vitro anti-inflammatory activities

Kirmizibekmez H., Inan Y., Reis R., Sipahi H., Goren A. C., Yesilada E.

NATURAL PRODUCT RESEARCH, vol.33, no.17, pp.2541-2544, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 17
  • Publication Date: 2019
  • Doi Number: 10.1080/14786419.2018.1448815
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2541-2544
  • Keywords: Clematis viticella, flavonoid glycosides, phenolic acids, hydroxytyrosol, anti-inflammatory activity, SUPPRESS TRYPTOPHAN BREAKDOWN, EXTRACTS
  • Acibadem Mehmet Ali Aydinlar University Affiliated: No


Phytochemical investigations on the EtOH extract of Clematis viticella led to the isolation of six flavonoid glycosides, isoorientin (1), isoorientin 3 '-O-methyl ether (2), quercetin 7-O-alpha-L-rhamnopyranoside (3), quercetin 3,7-di-O-alpha-L-rhamnopyranoside (4), manghaslin (5) and chrysoeriol 7-O-beta-D-glucopyranoside (6), one phenylethanol derivative, hydroxytyrosol (7), along with three phenolic acids, caffeic acid (8), (E)-p-coumaric acid (9) and p-hydroxybenzoic acid (10). The structures of the isolates were elucidated on the basis of NMR and HR-MS data. All compounds were isolated from C. viticella for the first time. Compounds 7 and 8 showed significant anti-inflammatory activity at 100 mu M by reducing the release of NO in LPS-stimulated macrophages comparable to positive control indomethacin. Compounds 3 and 7 exhibited anti-inflammatory activity through lowering the levels of TNF-alpha while 1, 3 and 5 decreased the levels of neopterin better than the positive controls.