Phenolic compounds from Hypericum calycinum and their antioxidant activity

Kirmizibekmez H., Bassarello C., Piacente S., Celep E., Atay I., Mercanoǧlu G., ...Daha Fazla

Natural Product Communications, cilt.4, sa.4, ss.531-534, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 4 Sayı: 4
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1177/1934578x0900400418
  • Dergi Adı: Natural Product Communications
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.531-534
  • Anahtar Kelimeler: Antioxidant activity, Butyl chlorogenate, Hypericaceae, Hypericum calycinum, Phenolic compounds
  • Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Hayır

Özet

From the MeOH extract of Hypericum calycinum, two caffeoylquinic acid derivatives, butyl chlorogenate (1), and chlorogenic acid (2), seven flavonoids, quercetin (3), quercitrin (4), hyperoside (5), isoquercitrin (6), miquelianin (7), rutin (8) and I3,II8-biapigenin (9) and two flavanols, (+)-catechin (10) and (-)-epicatechin (11) were isolated. Identification of the isolates was carried out by spectroscopic analysis including 1D and 2D NMR experiments as well as mass spectrometry. Free radical scavenging activities of the isolated compounds were determined in in-vitro 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (NO) scavenging models. The compounds showed strong DPPH and moderate NO scavenging activities in a concentration dependent manner. (+)-Catechin and (-)-epicatechin were found to be the most active compounds with IC50 values of 4.16 and 4.67 μM for DPPH and 190 and 170 μM for NO scavenging activities, respectively.