Akademik Veri Yönetim Sistemi
The 11th International Symposium on Medicinal and Aromatic Plants (MESMAP-11), Rimini, İtalya, 6 - 08 Mayıs 2025, ss.1, (Özet Bildiri)
Galega officinalis L. (Goat’s rue) from Fabaceae family is a perennial herb bearing white, blue or purple flowers. In medieval Europe, the plants aerial parts were medicinally used to treat various diseases such as plague and dysuria. Its hypoglycemic activity was discovered in the early 20th century.[1] Although there are studies investigating its bioactivities, evaluating the effect of human digestion on its phenolic profile and antioxidant activity is absent.[2] Studies show that human digestion system plays a crucial role on the bioavailability of herbal drugs.[3] Hence, this study aimed to compare the antioxidant activities of G. officinalis methanolic extracts before and after in vitro gastrointestinal human digestion simulation along with enlightening its phenolic profile. After the digestion simulation DPPH radical scavenging activity, metal reducing antioxidant activities (CUPRAC and FRAP), total antioxidant capacity (ToAC), total phenolic content (TPC), and total flavonoid content (TFC) assays were performed for each phase of digestion (ND: non-digested, PG: post-gastric, IN: serum-available/bioavailable). Additionally, phenolic profile of G. officinalis was brought to light with high performance thin-layer chromatography (HPTLC). Rutin and quercitrin presence were detected, therefore their absorbances were measured from TLC plate to obtain quantitative data. Results showed that overall antioxidant assays and TFC exhibited decreasing activity throughout the phases of digestion simulation. On the contrary, total phenolic content is increased after gastric digestion (ND: 110.87 ± 1.81 PG: 123.72 ± 1.68) yet serum-available fraction showed less activity compared to non-digested and post-gastric fractions (ND: 110.87 ± 1.81 IN: 87.77 ± 0.79). Rutin and quercitrin amounts were not significantly changed in PG fractions compared to ND extract whereas their amounts in IN fractions were not quantified. The obtained data displays the importance of digestion in terms of bioavailability of orally consumed herbal products and suggests that further studies should consider the effect of phytochemical profile on its bioavailability.