Aim: The shortage of cadaveric donors has sparked an interest in living donor transplantation in Eastern countries. Obtaining an optimal graft for a recipient and avoiding jeopardizing the health of a donor is the main concern in this procedure. Material and Method: A three-step assessment protocol was used to select the appropriate donor. The reasons for rejected candidates were analyzed to refine donor selection and minimise complications. Results: Between January 2016 and October 2017, 290 volunteers were evaluated for living liver donation, and 88 were rejected. At each step of donor evaluation, the individuals were excluded based on laboratory, imaging or liver biopsy results. Volumetric measurement results were the most detected reason (40.9%) to reject a candidate. Discussion: Ensuring donor safety in living donor liver transplantation is fundamental for transplant teams. Therefore, using systematic evaluation is the principal concern in this procedure.