REGULATION OF LYMPHOID-SPECIFIC IMMUNOGLOBULIN-MU HEAVY-CHAIN GENE ENHANCER BY ETS-DOMAIN PROTEINS

NELSEN B., TIAN G., ERMAN B., GREGOIRE J., MAKI R., GRAVES B., ...Daha Fazla

SCIENCE, sa.5117, ss.82-86, 1993 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 1993
  • Doi Numarası: 10.1126/science.8316859
  • Dergi Adı: SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.82-86
  • Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Hayır

Özet

The enhancer for the immunoglobulin mu heavy chain gene (IgH) activates a heterologous gene at the pre-B cell stage of B lymphocyte differentiation. A lymphoid-specific element, muB, is necessary for enhancer function in pre-B cells. A muB binding protein is encoded by the PU.1/Spi-1 proto-oncogene. Another sequence element, muA, was identified in the mu enhancer that binds the product of the ets-1 proto-oncogene. The muA motif was required for muB-dependent enhancer activity, which suggests that a minimal B cell-specific enhancer is composed of both the PU.1 and Ets-1 binding sites. Co-expression of both PU.1 and Ets-1 in nonlymphoid cells trans-activated reporter plasmids that contained the minimal mu enhancer. These results implicate two members of the Ets family in the activation of IgH gene expression.