3D Cancer Migration Assay with Schwann Cells


Fangmann L., Teller S., Stupakov P., Friess H., Ceyhan G. , Demir I. E.

SCHWANN CELLS: METHODS AND PROTOCOLS, vol.1739, pp.317-325, 2018 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 1739
  • Publication Date: 2018
  • Doi Number: 10.1007/978-1-4939-7649-2_20
  • Title of Journal : SCHWANN CELLS: METHODS AND PROTOCOLS
  • Page Numbers: pp.317-325

Abstract

In pancreatic cancer, neural invasion is one of the most common paths of cancer dissemination. Classically, cancer cells actively invade nerves and cause local recurrence and pain. Three-dimensional (3D) neural migration assay has become a standard tool for scientists to study neural invasion by confronting the involved cell types. This protocol introduces Schwann cells, i.e., the most prevalent cell type in peripheral nerves, in a novel heterotypic, glia-cancer-neuron, 3D migration assay for assessing their relevance in the early pathogenesis of neural invasion. Particularly, this assay allows the monitoring of the early Schwann cell migratory activity.