Akademik Veri Yönetim Sistemi
Anestezi Dergisi, cilt.11, sa.2, ss.147-150, 2003 (Scopus)
In our study we aimed to compare the effects of sigh and PEEP used for stabilization of alveoli during mechanical ventilation with low tidal volumes on histopathological structure and lipid peroxidation in healthy lung tissues. In this study, 16 healthy New Zealand albino rabbits were anaesthetized with ketamine. Tracheostomy was performed in all rabbits and ketamine was infused for maintenance via v. auricularis caudalis. The rabbits were randomly divided into two groups (n=8). Group I received low tidal volume and (5 mL kg-1) and sigh (twice in every 100 breaths, 1.5 times of the tidal volume) for three hours. Group II received low tidal volume (5 mL kg-1) and 10 cmH2O PEEP for three hours. Plasma MDA (malondialdehyde) levels were performed at the beginning of the study and at the end of third hour. The rabbits were sacrified at the end of the study. Lungs were then excised and kept with 10% formaldehit solution, and were stained with Haemotoxilyn-Eosine dye. Lungs were then evaluated for histopathological assessment under light microscopy and were scored between 0-4. The findings were analysed with Mann Whitney-U test. There was a significant increase in plasma MDA values in Group I as compared with Group II at the end of the third hour (p<0.05). Neutrophil infiltration and atelectatic areas were more severe in Group I than Group II (P<0.05). In conclusion, PEEP caused less histopathological changes and lipid peroxidation when was compared with sigh for alveolar stabilization.