Oligomer based real-time detection of microorganisms producing nuclease enzymes


Mozioglu E. , Akyurek S., Gunduz S., Akgoz M., Goren A. C. , Kocagoz T.

Analyst, vol.144, no.4, pp.1379-1385, 2019 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 144 Issue: 4
  • Publication Date: 2019
  • Doi Number: 10.1039/c8an02129e
  • Title of Journal : Analyst
  • Page Numbers: pp.1379-1385

Abstract

In this study, we provide a method using fluorescently labeled oligonucleotides for the diagnosis of microorganisms producing nucleases in real time, while growing them in culture media. The detection of such microorganisms was possible in a short period of time, as short as 10 minutes up to a maximum of 8 hours, depending on the bacterial density. We also showed the suitability of this new method for determination of minimum inhibitory concentration (MIC) in culture media in a very short period of time, compared to conventional methods. We believe that it can make a significant contribution to gain new insights for analysis of complex materials such as clinical samples, food samples and environmental samples.