CT10: A new cancer-testis (CT) antigen homologous to CT7 and the mage family, identified by representational-difference analysis


Güre A. O. , Stockert E., Arden K. C. , Boyer A. D. , Viars C. S. , Scanlan M. J. , ...More

International Journal of Cancer, vol.85, no.5, pp.726-732, 2000 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 85 Issue: 5
  • Publication Date: 2000
  • Title of Journal : International Journal of Cancer
  • Page Numbers: pp.726-732

Abstract

Assays relying on humoral or T-cell-based recognition of tumor antigens to identify potential targets for immunotherapy have led to the discovery of a significant number of immunogenic gene products, including cancer-testis (CT) antigens predominantly expressed in cancer cells and male germ cells. The search for cancer-specific antigens has been extended via the technique of representational-difference analysis and SK-MEL-37, a melanoma cell line expressing a broad range of CT antigens. Using this approach, we have isolated CT antigen genes, genes over-expressed in cancer, e.g., PRAME and KOC, and genes encoding neuro-ectodermal markers. The identified CT antigen genes include the previously defined MAGE-A6, MAGE-A4a, MAGE-A10, CT7/MAGE- CI, as well as a novel gene designated CT10, which shows strong homology to CT7/MAGE-CI both at cDNA and at genomic levels. Chromosome mapping localized CT10 to Xq27, in close proximity to CT7/MAGE-CI and MAGE-A genes. CT10 mRNA is expressed in testis and in 20 to 30% of various human cancers. A serological survey identified 2 melanoma patients with anti-CT10 antibody, demonstrating the immunogenicity of CT10 in humans. (C) 2000 Wiley-Liss, Inc.