TRAIL conjugated silver nanoparticle synthesis, characterization and therapeutic effects on HT-29 colon cancer cells


Birtekocak F., Demirbolat G. M. , Cevik O.

Iranian Journal of Pharmaceutical Research, vol.20, no.2, pp.45-56, 2021 (Journal Indexed in SCI Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 2
  • Publication Date: 2021
  • Doi Number: 10.22037/ijpr.2020.112069.13514
  • Title of Journal : Iranian Journal of Pharmaceutical Research
  • Page Numbers: pp.45-56
  • Keywords: Apoptosis, Colon cancer, HT-29 cells, Silver nanoparticles, TRAIL

Abstract

© 2021, Iranian Journal of Pharmaceutical Research. All rights reserved.Colon cancer is one of the most prominent causes of cancer-related morbidity and mortality and curable if detected in the early stages. TNF-related apoptosis-inducing ligand (TRAIL) is a therapeutic protein and has a potential anti-cancer activity that is widely used for the treatment of several cancers. In this study, we aimed to develop a silver nanoparticle system conjugated with TRAIL and coated with PEG (AgCTP NPs) to improve the therapeutic effects of colon cancer. AgCTP NPs were characterized by UV spectrum, FTIR and zetasizer. Cytotoxicity, hemolysis assay and apoptotic effects of nanoparticles were investigated using a colon cancer cell line (HT-29) in-vitro. Treatment with AgCTP NPs effectively inhibited proliferation and colony formation of HT-29 cells. The apoptotic effects of nanoparticles on HT-29 cells were determined as Bax, Bcl-2, PARP and clv-PARP protein expression levels using Western blot. Apoptotic proteins were upregulated by AgCTP NPs. In this study, we demonstrated that AgCTP NPs had an anti-cancer effect by activating cell death. Thus, we have confirmed that silver nanoparticles can be selected as a good carrier for TRAIL therapeutic proteins that can be used to treat colon cancer.