Effects of Neuronal Membrane Precursors Administered During Prenatal and Lactation Periods on Skeletal Muscle in the 5XFAD Alzheimer's Animal Model
17th International Symposium of Clinical and Applied Anatomy, Vienna, Austria, 3 - 05 September 2026, vol.267, pp.111-112, (Summary Text)
- Publication Type: Conference Paper / Summary Text
- Volume: 267
- Doi Number: 10.1016/j.aanat.2026.153087
- City: Vienna
- Country: Austria
- Page Numbers: pp.111-112
- Acibadem Mehmet Ali Aydinlar University Affiliated: Yes
Abstract
Objective: Alzheimer’s disease (AD) is primarily characterized by neurodegeneration; however, emerging evidence indicates that peripheral tissues, including skeletal muscle, are also affected. This study aimed to evaluate structural and metabolic alterations in skeletal muscle tissue in AD and to investigate the potential effects of a supportive diet. Methods and Materials: A total of 12 5xFAD transgenic mice, a well-established AD model, were included. During prenatal and lactation periods, animals received a combined diet containing uridine monophosphate (UMP), choline, and fish oil. At 9 months 17th International Symposium of Clinical and Applied Anatomy – Conference Proceedings Annals of Anatomy 267 (2026) 152887 111 of age, mice were divided into four groups: CTg- (control nontransgenic, n=3), CTg+ (control transgenic, n=3), ETg- (diet nontransgenic, n=3), and ETg+ (diet transgenic, n=3). Histopathological evaluation of m. gastrocnemius was performed using H&E and PAS staining, and mitochondrial activity was assessed by COXIV immunohistochemistry. Results: The CTg+ group showed the most pronounced muscle degeneration and atrophy, with reduced fiber diameter. In contrast, diet-treated groups (ETg−, ETg+) demonstrated increased fiber diameter and glycogen content (p<0.05). PAS staining revealed higher glycogen levels in diet-treated groups than controls. COXIV immunoreactivity was significantly reduced in the CTg+ group compared to the CTg− and ETg+ groups, while COXIV expression remained preserved in the ETg+ group (p<0.05). Conclusion: These findings suggest that a supportive diet containing UMP, choline, and fish oil may exert protective and restorative effects against structural and metabolic impairments in skeletal muscle associated with AD.