Akademik Veri Yönetim Sistemi
Conference on PhD Research in Microelectronics and Electronics, İstanbul, Türkiye, 22 - 25 Haziran 2008, ss.25-28
This paper presents two affinity-based biosensor implementations for the detection and quantification of cardiovascular risk proteins/markers, such as C-Reactive Protein, Myoglobin, TNF-alpha, Serum Amyloid, using specific antibodies. The first method is based on interdigitated microelectrodes while the other is based microarrays. The signal transduction in micro-IDEs from biological-signal to the electronic/electrical signal occurs via fringe fields and dielectric change, induced by the capture of antigens by already immobilized antibody between the microelectrodes. Being process/integration-compatible with microelectronic/fabrication technologies, this method provides many advantages for the utilization in portable and/or remote sensing applications, such as smaller size, lower power. consumption, small sample consumption, disposability, etc. The other method also analyzes the capability and reliability of sandwich-type antibody microarrays for the detection quantification of cardiovascular risk markers, using optical scanner system for the detection of fluorescent-labeled, sandwiched-structured antibodies on the chemically modified glass substrates. Our findings in microarray study provides the advantages for lab-based detection and quantification applications such as being able to detect and quantify multiple markers at once and also better dynamic ranges over some of the commercial ELISA testkits.