Calcium signaling is required for ultrasound-stimulated aggrecan synthesis by rat chondrocytes

Parvizi J., Parpura V., Greenleaf J., Bolander M.

JOURNAL OF ORTHOPAEDIC RESEARCH, cilt.20, sa.1, ss.51-57, 2002 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 1
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1016/s0736-0266(01)00069-9
  • Dergi Adı: JOURNAL OF ORTHOPAEDIC RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.51-57
  • Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Hayır

Özet

Low-intensity ultrasound accelerates fracture healing in humans. In rat femur fracture models, ultrasound advanced healing is associated with increased proteoglycan expression. Here we report that ultrasound stimulation of primary rat chondrocytes elevated the intracellular concentration of calcium [Ca2+](i.) The [Ca2+](j) increase was rapid and transient at lower pressures (175-320 kPa), but rapid and sustained at higher ultrasound exposures (350-500 kPa). Chelating internal [Ca2+](i) with 1,2-bis(2-aminophenoxy) ethane-N-N-N',N'- tetraacetic acid (BAPTA-AM), stopping the Ca2+/ATP-ase induced mitochondrial release of [Ca2+](i) with Thapsigargin, or removing [Ca2+](i) from the medium with EGTA inhibited the stimulatory effects of ultrasound on proteoglycan synthesis. These results imply that ultrasound-stimulated synthesis of cell matrix proteoglycan, associated with accelerated fracture healing, is mediated by intracellular calcium signaling. (C) 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.