Characterization of carotid endothelial cell proliferation on Au, Au/GO, and Au/rGO surfaces by electrical impedance spectroscopy

Simsek F., Can O. M., Garipcan B., Kocaturk O., Ulgen Y.

MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING, sa.7, ss.1431-1443, 2020 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s11517-020-02166-0
  • Dergi Adı: MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, ABI/INFORM, Agricultural & Environmental Science Database, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, Business Source Elite, Business Source Premier, CINAHL, Compendex, Computer & Applied Sciences, EMBASE, INSPEC, MEDLINE
  • Sayfa Sayıları: ss.1431-1443
  • Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Hayır

Özet

To the best of the authors' knowledge, testing the biocompatibility of graphene coatings can be considered as the first to demonstrate human carotid endothelial cell (HCtAEC) proliferation on Au, graphene oxide-coated Au (Au/GO), and reduced graphene oxide-coated Au (Au/rGO) surfaces. We hypothesized that stent material modified with graphene (G)-based coatings could be used as electrodes for electrical impedance spectroscopy (EIS) in monitoring cell cultures, i.e., endothelialization. Alamar Blue cell viability assay and cell staining and cell counting with optical images were performed. For EIS analysis, an EIS sensor consisting of Au surface electrodes was produced by the photolithographic technique. Surface characterizations were performed by considering scanning electron microscope (SEM) and water contact angle analyses. Results showed that GO and rGO coatings did not prevent neither the electrical measurements nor the cell proliferation and that rGO had a positive effect on HCtAEC proliferation. The rate of increase of impedance change from day 1 to day 10 was nearly fivefold for all electrode surfaces. Alamar Blue assay performed to monitor cell proliferation rates between groups, and rGO has shown the highest Alamar Blue reduction value of 43.65 +/- 8.79%.