Objective: New disinfectants are being developed due to the emerging resistance of bacterial spores, mycobacteriae, Gram negative bacilli, Pseudomonas and staphylococci species to routine antiseptics and disinfectants like quaternary ammonium compounds, hydrogen peroxides, peracetic acid,phenol compounds and gluteraldehyde. These new agents are superior to others for their effectiveness at low consentrations and are safely used without adverse effects in surface and human disinfection. Material and Methods: In this study, the effectiveness against the standard strains Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 15442, Candida albicans ATCC 90028 of 70% ethyl alcohol, 0.5% sodium hypochlorite, 2% gluteraldehyde, 1% benzalkonium chloride and two new disinfectants including 25% Akacid (R) forte and 15000 ppm Oxel-B (R) has been studied. 5 minutes and 30 minutes have been taken as a contact time. For the standardization, as a Turkish guideline TS EN 1040 (1999) based on EN 1040 (1997) has been used. Results: It has been found that sodium hypochlorite, Akacid forte and Oxel-B are more effective at low consentrations also at both two contact times of 5 min and 30 min. The other disinfectants were found as effective at the suggested concentrations. Conclusion: In the highest level disinfection, use of disinfection killing all microorganisms except bacteria spores is targeted; because the resistance to these disinfectants used for this reasons has been rapidly increased, new agents are being developed. For these two new disinfectants, especially for Oxel-B to make forward studies will effect to find broad fields to use. To suspect the superiority of the new developed disinfectants will play a role in disinfectant policy in our hospitals.