Detection of Listeria monocytogenes in milk by the polymerase chain reaction

Ahrabi, SS; Koseoglu, O; Kocagoz, ZÜHTÜ; Erguven, S; Gunalp, A

doi:10.1016/s0934-8840(99)80120-0

Detection of Listeria monocytogenes in milk by the polymerase chain reaction

Ahrabi S., Koseoglu O., Kocagoz T., Erguven S., Gunalp A.

ZENTRALBLATT FUR BAKTERIOLOGIE-INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY VIROLOGY PARASITOLOGY AND INFECTIOUS DISEASES, cilt.289, sa.1, ss.31-36, 1999 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 289 Sayı: 1
Basım Tarihi: 1999
Doi Numarası: 10.1016/s0934-8840(99)80120-0
Dergi Adı: ZENTRALBLATT FUR BAKTERIOLOGIE-INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY VIROLOGY PARASITOLOGY AND INFECTIOUS DISEASES
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
Sayfa Sayıları: ss.31-36
Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Hayır

Özet

A on the polymerase chain reaction (PCR) method based was developed for detection of Listeria monocytogenes in milk samples after enrichment culture. It consists of culturing samples in Listeria enrichment broth, followed by DNA extraction and detection of the organism using PCR. Dilutions of L. monocytogenes in milk were subjected to PCR amplification after enrichment culture. When determing the sensitivity of the method, it was found to be possible to detect 37 CFU (colony forming unit gl/ml) of the bacterium in milk. The method was assessed as a sensitive, specific, times-saving and practical way of detecting L. monocytogenes in milk samples.