Construction of a 3D printed, human gingival MSC seeded alveolar bone implant
Journal of Applied Biomaterials and Functional Materials, cilt.24, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 24
- Basım Tarihi: 2026
- Doi Numarası: 10.1177/22808000261452915
- Dergi Adı: Journal of Applied Biomaterials and Functional Materials
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Compendex, EMBASE, INSPEC, MEDLINE, Directory of Open Access Journals, Academic Search Ultimate (EBSCO), Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest), Materials Science & Engineering Collection (ProQuest), Technology Collection (ProQuest)
- Anahtar Kelimeler: 3D printing, alveolar bone augmentation, bone morphogenetic protein 2, dental tissue engineering, mesenchymal stem cells
- Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Evet
Özet
Background/objective(s)/introduction: Tissue engineered scaffolds fabricated by 3D printing promises to enhance the success rate in alveolar bone augmentation. The aim of this study was to construct and characterize a 3D printed poly(ε-caprolactone; PCL) scaffolds treated with bone morphogenetic protein 2 (BMP-2) and carrying human gingival mesenchymal stem cells (hGMSCs) for potential use in the augmentation of alveolar bone. Materials and methods: PCL scaffolds with defined pore geometry were fabricated by 3D printing using fused deposition modeling (FDM) and treated with O2 plasma to enhance BMP-2 and cell adhesion. The scaffolds were characterized by compression testing, scanning electron microscopy (SEM), and water contact angle measurement. BMP-2 was bound to the surface, and hGMSCs from gingival connective tissue were seeded onto the scaffolds. BMP-2 was quantified by elisa. The cells were identified as stem cells based on their differentiation capacity and immunophenotypic profile. Osteogenic differentiation on BMP-2 bound scaffolds was assessed using alkaline phosphatase (ALP) activity and calcium deposition assays. Results: Compressive modulus of the PCL scaffolds (73.2 ± 17.1 MPa) was close to human alveolar bone (96.2 ± 40.6 MPa). SEM revealed a porous design with continuous struts, creating an average pore size of 400 µm and zig-zag channels suitable for cell infiltration and adhesion. O2 plasma treatment decreased the water contact angle from 110° ± 4° to 59° ± 2°, indicating improved surface hydrophilicity. ELISA showed that over 98% of BMP-2 remained bound to the scaffold for 28 days, indicating prolonged growth factor presence during cell culture. Within the environment created, BMP-2 bound scaffolds led to a threefold increase in ALP activity and higher calcium deposition compared to controls, confirming enhanced osteogenic differentiation. Conclusion(s): 3D printed PCL scaffolds functionalized with BMP-2 and seeded with hGMSCs exhibited highly improved in vitro osteogenic properties which promises to improve alveolar bone augmentation results.