Akademik Veri Yönetim Sistemi
JOURNAL OF CRANIOFACIAL SURGERY, cilt.21, sa.6, ss.1745-1749, 2010 (SCI-Expanded)
Objectives: To determine the possible role of oxidants and antioxidants in the pathogenesis of in laryngeal squamous cell carcinoma. Design and Setting: Our study involved patients with newly diagnosed laryngeal cancer (n = 29) and same age-and sex-matched healthy individuals (n = 21). Serum malondialdehyde (MDA) and paraoxonase (PON1) levels were measured by colorimetric methods and 8-hydroxy-2'-deoxyguanosine (8-OH-dG) was measured using enzyme-linked immunosorbent assay in fasting blood samples of participants. Results: The levels of 8-OH-dG (control, 4.61 +/- 1.27 ng/mL; patient, 11.70 +/- 2.44 ng/mL; P < 0.001) and MDA (control, 4.16 +/- 1.02 nmol/mL; patient, 8.74 +/- 1.65 nmol/mL; P < 0.001) were significantly higher, and those of PON1 (control, 170.86 +/- 72.46 U/mL; patient, 80.44 +/- 29.81 U/mL; P G 0.001) were significantly lower in patients. There were no statistically significant differences in the 8-OH-dG, MDA levels, and PON1 activity in relation to T (tumor) staging of differentiation and different smoking/drinking status. There was a statistically significant difference in MDA levels (10.24 +/- 0.64 nmol/mL) only in stage II laryngeal cancer. There were a statistically significant positive correlation between serum MDA and 8-OH-dG (r = 0.887, P < 0.001), a statistically significant negative correlation between serum MDA and serum PON1 (r = -0.477, P < 0.01), and a statistically significant negative correlation between serum 8-OH-dG and serum PON1 in patients (r = -0.420, P < 0.05). Conclusions: We conclude that, in patients with laryngeal squamous cell carcinoma, the oxidant/antioxidant balance was impaired in favor of lipid peroxidation and DNA damage.