Neural invasion in pancreatic cancer: A mutual tropism between neurons and cancer cells

Ceyhan G., Demir I. E., Altintas B., Rauch U., Thiel G., Mueller M. W., ...Daha Fazla

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, cilt.374, sa.3, ss.442-447, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 374 Sayı: 3
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1016/j.bbrc.2008.07.035
  • Dergi Adı: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.442-447
  • Anahtar Kelimeler: neural invasion, pancreatic cancer, in-vitro migration, myenteric plexus, dorsal root ganglia, NGF, GDNF, artemin, persephin, neurturin, PERINEURAL INVASION, MYENTERIC PLEXUS, EXPRESSION, GROWTH
  • Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Hayır

Özet

Neural invasion by pancreatic cancer cells (PCC) worsens the prognosis and frequently limits curative resection. We established a novel in-vitro model in which T3M4-PCCs were co-cultured with either isolated myenteric plexus cells (MP) or dorsal root ganglia (DRG) of newborn rats within a three-dimensional extracellular matrix gel. The close vicinity of MP or DRG to T3M4-PCCs induced early morphologic changes on T3M4-PCCs at the migration front prior to the migration process with elongated and neurite-targeting PCCs, compared to round and non-grouping at the non-migrating front. T3M4-PCCs built cancer-cell clusters around the DRG or MP, a process which was accelerated by increasing number of T3M4-PCCs or neurons. These findings indicate that neuro-cancer interactions start prior to PCC migration and induce evident changes in cancer and nerve biology. These findings can be reproduced within the introduced 3D in-vitro migration assay which allows investigation in the early pathogenesis of neural PCC invasion. (C) 2008 Elsevier Inc. All rights reserved.