Hypercoagulant Effects of UDP via Leukocytes

Sağdilek E., Kılınç E.

TBS International Biochemistry Congress 2021-32nd National Biochemistry Congress, Gaziantep, Turkey, 27 - 30 October 2021, pp.61

  • Publication Type: Conference Paper / Summary Text
  • City: Gaziantep
  • Country: Turkey
  • Page Numbers: pp.61


BACKGROUND AND AIM: P2Y12 receptor is stimulated by ADP and, platelets are activated. This receptor has been shown to be inhibited by UTP. It has been shown that UDP plays a role in platelet-leukocyte interaction via P2Y14 receptor and has no effect on hemostasis. Effects of uridine nucleotides on hemostasis via different purinergic receptors are not yet fully known. METHODS: Blood samples from volunteers, as whole blood and platelet-rich plasma (PRP), were used in experiments to investigate the role of UDP and UTP in hemostasis. Platelet aggregation tests showed that UTP and UDP inhibited ADP-induced platelet aggregation. RESULTS: Thromboelastogram experiments were performed in whole blood. While UTP showed a hypocoagulant effect as if supporting the finding in aggregation, UDP showed a hypercoagulant effect on the contrary. In thromboelastogram experiments with PRP, both UTP and UDP showed hypocoagulant effect. CONCLUSIONS: Based on UTP antagonism of P2Y12 receptors, both its inhibition in aggregometry and thromboelastogram can be understood. Inhibition of ADP-induced platelet aggregation by UDP in the aggregometer can be considered through P2Y12 antagonism. Experiments with PRP in the thromboelastogram support this finding. However, hypercoagulant effect of UDP in whole blood on thromboelastogram can be explained through P2Y14 receptors. It is known that UDP also stimulates P2Y6 and P2Y14 receptors on leukocytes. Stimulation of these receptors increases leukocyte chemotaxis and NET formation. The occurrence of platelet-leukocyte interaction by activating P2Y14 receptors with UDP may explain the hypercoagulant effect. UDP exerts its hypercoagulant effect in whole blood through the platelet leukocyte interaction and may be important in prothrombotic processes.