DEVELOPMENT of LIPOSOMAL CARRIER SYSTEMS of HYDROLYZED COLLAGEN, RESVERATROL, RETINOL and INVESTIGATION of THEIR PENETRATIONS

---

Thesis Type: Postgraduate

Institution Of The Thesis: Yeditepe University, Faculty Of Pharmacy, Department Of Pharmaceutıcal Technology, Turkey

Approval Date: 2020

Thesis Language: English

Student: Hakan Sevinç

Supervisor: Yasemin Uzuner

Abstract:

Sevinç, H. (2020), “Development of Liposomal Carrier Systems of Hydrolyzed
Collagen, Resveratrol, Retinol, and investigation of Their Penetrations.” Yeditepe
University, Institute of Health Sciences, Department of Pharmaceutical Technology,
MSc Thesis, İstanbul.
In recent years, not only for cosmetic products but also for the administration of drugs,
transdermal delivery is becoming an important route of administration. Percutaneous
permeation of molecules is controlled by the diffusion laws and is a big challenge in
biomedical sciences. Transdermal delivery systems have many advantages such as
avoidance of the first-pass metabolism, sustained-release opportunity and less frequent
dosing, ease of application, and better user compliance.
Ethosome is one of these new and promising delivery systems, which is a new generation
of liposome and because of the presence of alcohol in its structure, it offers an increased
solubility for hydrophilic, hydrophobic and amphiphilic active materials. Also, the
smaller and more flexible structure makes ethosome a better carrier than liposome.
Resveratrol, retinol and hydrolyzed collagen ethosomes were prepared as a possibility of
developing new delivery systems with these anti-aging ingredients. The results of the
studies showed that the ethosomes were easy to produce and stable at the duration of the
tests (180 days at 25ºC±2ºC), no phase separation or bottom precipitation was observed;
the changes in the measurements of particle size, size distribution, poly-dispersity index,
zeta potential, pH, density, and conductivity were within acceptable intervals. The
presence of alcohol increased the solubility of the ingredients resulting in good
entrapment of respective active materials in ethosomes at a minimum of 86 % and
retained the entrapped actives at a minimum of 71% level after 180 days. İn-vitro release
studies by Franzcell diffusion system were performed with the ethosome formulations
using two different synthetic membranes; Strat-M® (layered skin mimicking) and 12-
14KD cellulose acetate dialysis membrane. The release rates of the actives from
ethosomes were compared with the simple solutions of the actives at 1,5%. The results
clearly showed that ethosomes released the actives in a sustained release manner and the
release rates were slower when Strat-M® was used.
Keywords: Retinol, hydrolyzed collagen, resveratrol, liposomes, ethosomes, in-vitro
tests, franzcell